DeepDxTM Monkeypox Virus Real-Time PCR Kit
Catalog Number
SG1853M
Request Quote
First Name *
Last Name *
Email Address *
Instiution *
Prodcuts/Services *
Questions & Comments *
Download
DeepDxTM Monkeypox Virus Real-Time PCR Kit

DeepDxTM Monkeypox Virus Real-Time PCR Kit

Catalog Number

SG1853M

Description

TheDeepDxTM Monkeypox Virus (MPXV) PCR Kit is a real-time PCR assay forthe detection of MPXV DNA in a liquid sample. It uses a fluorescent probe withspecific primer sets to detect the J2R gene specific for the genome of MPXV.

Sensitivity

4copies / µl

Specificity

Doesnot cross react with cowpox, camelpox or vaccinia virus. Features dUTP-UDGsystem to prevent carry-over contamination.

Number of Targets Detected

1

Species Detected

Virus

Compatible Sample Types

DNA

Method of Detection

PCR

Quantitative/Semi-Quantitative

Qualitative

Product Specifications

Size

96tests

Protein Information

Accession Number

O57108

Gene Symbols

J2R

Protein Name & Synonyms

J2R(J2L)

Introduction

Monkeypoxvirus is a virus from the orthopox family of viruses, a family most oftenassociated with the more severe smallpox virus (variola), or the origination ofvaccinations from the cowpox virus. Originally named for an outbreak among monkeys,it was first identified in humans in central and western Africa around 1970where it continues to circulate at a low endemic level. While the virus isclinically less severe than smallpox, the symptoms are similar. Withvaccinations against smallpox ceasing in 1972 and the eradication of thesmallpox virus, vaccinations in the younger population (<50 years of age) isextremely limited. The escape from the endemic regions has the World HealthOrganization on alert given the lack of associated travel within the region ofthose suspected and confirmed cases. Understanding how the virus is beingspread, the populations it is spreading to, and the potential immune responsesagainst the virus are of increasing importance in the research field.

ThisPCR kit is used for the qualitative in vitro detection of Monkeypox virus(MPXV) nucleic acid from purified DNA samples. Sample DNA should be purifiedaccording to manufacturer or related procedures.

Product Features

Monkeypoxvirus specific / no cross-reacitvity with other Orthopox viruses.

Hot-startTaq DNA Polymerase with high efficiency and fidelity.

Doublequencher probe ensures enhanced specificity.

UDGand dUTP system minimizes carry-over contamination.

ROXreference dye improves accuracy and reproducibility.

Kit Components

Component

Ingredients

Specification

Quantity

2x PCR Reaction Solution

Buffer, dNTP’s, enzyme, ROX reference dye

1000 µL / tube

1 tube

Primers and Probe

Oligonucleotides

500 μl / tube

1 tube

Positive Control

Synthetic Monkeypox Virus Genomic DNA

50 µL / tube

1 tube

Negative Control

Nuclease-free water

500 µL / tube

1 tube

Storage/Stability

The kit is shipped on dry ice and can be stored at -20°C for a period of 12 months prior to opening. After opening, the reagents are valid for at least 6 months if stored at -20°C.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

DeepDxTM Monkeypox Virus Real-Time PCR Kit

Catalog Number

SG1853M

Description

TheDeepDxTM Monkeypox Virus (MPXV) PCR Kit is a real-time PCR assay forthe detection of MPXV DNA in a liquid sample. It uses a fluorescent probe withspecific primer sets to detect the J2R gene specific for the genome of MPXV.

Sensitivity

4copies / µl

Specificity

Doesnot cross react with cowpox, camelpox or vaccinia virus. Features dUTP-UDGsystem to prevent carry-over contamination.

Number of Targets Detected

1

Species Detected

Virus

Compatible Sample Types

DNA

Method of Detection

PCR

Quantitative/Semi-Quantitative

Qualitative

Product Specifications

Size

96tests

Protein Information

Accession Number

O57108

Gene Symbols

J2R

Protein Name & Synonyms

J2R(J2L)

Introduction

Monkeypoxvirus is a virus from the orthopox family of viruses, a family most oftenassociated with the more severe smallpox virus (variola), or the origination ofvaccinations from the cowpox virus. Originally named for an outbreak among monkeys,it was first identified in humans in central and western Africa around 1970where it continues to circulate at a low endemic level. While the virus isclinically less severe than smallpox, the symptoms are similar. Withvaccinations against smallpox ceasing in 1972 and the eradication of thesmallpox virus, vaccinations in the younger population (<50 years of age) isextremely limited. The escape from the endemic regions has the World HealthOrganization on alert given the lack of associated travel within the region ofthose suspected and confirmed cases. Understanding how the virus is beingspread, the populations it is spreading to, and the potential immune responsesagainst the virus are of increasing importance in the research field.

ThisPCR kit is used for the qualitative in vitro detection of Monkeypox virus(MPXV) nucleic acid from purified DNA samples. Sample DNA should be purifiedaccording to manufacturer or related procedures.

Product Features

Monkeypoxvirus specific / no cross-reacitvity with other Orthopox viruses.

Hot-startTaq DNA Polymerase with high efficiency and fidelity.

Doublequencher probe ensures enhanced specificity.

UDGand dUTP system minimizes carry-over contamination.

ROXreference dye improves accuracy and reproducibility.

Kit Components

Component

Ingredients

Specification

Quantity

2x PCR Reaction Solution

Buffer, dNTP’s, enzyme, ROX reference dye

1000 µL / tube

1 tube

Primers and Probe

Oligonucleotides

500 μl / tube

1 tube

Positive Control

Synthetic Monkeypox Virus Genomic DNA

50 µL / tube

1 tube

Negative Control

Nuclease-free water

500 µL / tube

1 tube

Storage/Stability

The kit is shipped on dry ice and can be stored at -20°C for a period of 12 months prior to opening. After opening, the reagents are valid for at least 6 months if stored at -20°C.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.