Yeast Two-hybrid Service
  • OVERVIEW


Yeast Two-hybrid Service


Yeast two-hybrid system is a technology for identifying and detecting protein interactions. It has been used in many research fields because of its high sensitivity, powerful and wide application range.


Nucleoprotein Yeast Two-Hybrid

The nucleoprotein yeast two-hybrid technique was originally established by Fields et al. to study the properties of the yeast transcription factor GAL4. After continuous improvement, it has been developed into a mature protein-protein interaction research tool. The characteristics of the real situation of intracellular interactions are widely used in the screening of interacting proteins, the identification and validation of protein interactions, the exploration of protein interaction mechanisms, and the drawing of protein linkage maps.


Membrane Protein Yeast Two-Hybrid

Based on the traditional yeast two-hybrid system, DUALmembrane technology uses the separated ubiquitin system (split-ubiquitin) to screen protein interactions. Ubiquitin, as a degradation signal molecule, is artificially divided into two parts: N-terminus (Nub), C-terminal (Cub), complementary remodeled intact ubiquitin molecules can be recognized by ubiquitin-specific proteases (UBPs), resulting in enzymatic cleavage of ubiquitin-linked proteins.


Applications in Multiple Fields

Plants: Mechanisms of Self-Incompatibility Studies...

Virus: prion protein, virus protein interacting protein screening...

Signaling Pathways: Transcription Factors, Estrogen Alpha Receptor Interacting Protein Screening...

Cancer Medicine: Screening of Cancer-Related Proteins, Apoptosis-Related Protein-Interacting Proteins...

Parasitic medicine: pathogenic mechanism research of virulence factors, screening of galectin-interacting proteins...

Basic research: myosin, proteasome regulator interacting protein screening, fertilized egg development...


Highlights of Our Services

-The method is convenient and quick to obtain reliable experimental conclusions.

-Years of library construction experience. Four optimized Total RNA extraction technology solutions can effectively ensure the purity and integrity of Total RNA and ensure the diversity of samples.

-The efficient SM-directed triple-frame library construction technology can ensure that the positive rate of the library is more than 98%, and one cDNA sample is connected to three different reading frame vectors, which can easily achieve zero differences in the quality control indicators of the triple-frame library and reduce library differences. impact on screening results.

-The pPR3-N series vector is directionally transformed into pPR3-GW vector, which can easily realize the one-step LR reaction from the nuclear protein library to the membrane protein library and obtain the membrane protein yeast two-hybrid library.


Scope of Our Service

Yeast Two Hybrid Library Construction

-Receipt of orders and samples

-Total RNA extraction mRNA purification

-Reverse transcription, double-stranded cDNA synthesis and purification

-Co-transfer to yeast competent cells

-Transformation efficiency measurement

-Library titer measurement

-Delivery of the library and project report


Yeast Two-Hybrid Library Screening

-Receipt of orders and samples

-Construction of bait vector

-Toxicity detection

-Determination of hybridization efficiency

-Identification of positive clones

-Results delivery


Sample Requirements

Service

Sample

Deliverables

Library Construction

Tissue/cell

Yeast library strain with titer above 1×108.

Picture of library PCR identification results (positive rate is about 90%).

Library Screening

Protein Sequence/Gene Sequence/Plasmid

All original pictures during the screening process.

All interacting prey protein sequences.



Yeast Two-hybrid Service


Yeast two-hybrid system is a technology for identifying and detecting protein interactions. It has been used in many research fields because of its high sensitivity, powerful and wide application range.


Nucleoprotein Yeast Two-Hybrid

The nucleoprotein yeast two-hybrid technique was originally established by Fields et al. to study the properties of the yeast transcription factor GAL4. After continuous improvement, it has been developed into a mature protein-protein interaction research tool. The characteristics of the real situation of intracellular interactions are widely used in the screening of interacting proteins, the identification and validation of protein interactions, the exploration of protein interaction mechanisms, and the drawing of protein linkage maps.


Membrane Protein Yeast Two-Hybrid

Based on the traditional yeast two-hybrid system, DUALmembrane technology uses the separated ubiquitin system (split-ubiquitin) to screen protein interactions. Ubiquitin, as a degradation signal molecule, is artificially divided into two parts: N-terminus (Nub), C-terminal (Cub), complementary remodeled intact ubiquitin molecules can be recognized by ubiquitin-specific proteases (UBPs), resulting in enzymatic cleavage of ubiquitin-linked proteins.


Applications in Multiple Fields

Plants: Mechanisms of Self-Incompatibility Studies...

Virus: prion protein, virus protein interacting protein screening...

Signaling Pathways: Transcription Factors, Estrogen Alpha Receptor Interacting Protein Screening...

Cancer Medicine: Screening of Cancer-Related Proteins, Apoptosis-Related Protein-Interacting Proteins...

Parasitic medicine: pathogenic mechanism research of virulence factors, screening of galectin-interacting proteins...

Basic research: myosin, proteasome regulator interacting protein screening, fertilized egg development...


Highlights of Our Services

-The method is convenient and quick to obtain reliable experimental conclusions.

-Years of library construction experience. Four optimized Total RNA extraction technology solutions can effectively ensure the purity and integrity of Total RNA and ensure the diversity of samples.

-The efficient SM-directed triple-frame library construction technology can ensure that the positive rate of the library is more than 98%, and one cDNA sample is connected to three different reading frame vectors, which can easily achieve zero differences in the quality control indicators of the triple-frame library and reduce library differences. impact on screening results.

-The pPR3-N series vector is directionally transformed into pPR3-GW vector, which can easily realize the one-step LR reaction from the nuclear protein library to the membrane protein library and obtain the membrane protein yeast two-hybrid library.


Scope of Our Service

Yeast Two Hybrid Library Construction

-Receipt of orders and samples

-Total RNA extraction mRNA purification

-Reverse transcription, double-stranded cDNA synthesis and purification

-Co-transfer to yeast competent cells

-Transformation efficiency measurement

-Library titer measurement

-Delivery of the library and project report


Yeast Two-Hybrid Library Screening

-Receipt of orders and samples

-Construction of bait vector

-Toxicity detection

-Determination of hybridization efficiency

-Identification of positive clones

-Results delivery


Sample Requirements

Service

Sample

Deliverables

Library Construction

Tissue/cell

Yeast library strain with titer above 1×108.

Picture of library PCR identification results (positive rate is about 90%).

Library Screening

Protein Sequence/Gene Sequence/Plasmid

All original pictures during the screening process.

All interacting prey protein sequences.


Request Quote
First Name *
Last Name *
Email Address *
Instiution *
Prodcuts/Services *
Questions & Comments *