Western Blot Services
  • OVERVIEW

Western Blot Services


WB (Western blot), namely Western blotting or immunoblotting, is a technique widely used in molecular biology, biochemistry, immunology, and many other fields. The method involves using gel electrophoresis to separate the sample's proteins. The separated proteins are transferred out of the gel to the surface of a membrane. The membrane is exposed to an antibody specific to the target protein. Binding of the antibody is detected using a radioactive or chemical tag.

WB technology has the high resolution of SDS-PAGE and the high specificity and sensitivity of solid-phase immunoassays. The selection of a suitable internal reference antibody can correct the errors in protein quantification and sample loading, and the protein expression in different samples can be qualitatively or quantitatively analyzed by grayscale measurement.

Seattle Genova provides WB detection technology services, providing conclusive evidence of the expression level of encoded genes, which can be used for specific gene regulation and gene function research, and can also be used as a verification experiment for protein chips and protein profiles.


Workflow of our service

-Receipt of orders and samples

-Protein concentration determination

-Electrophoretic transfer

-Hybridization

-Chromogenic data analysis

-Result delivery


Sample requirements

Tissue or cell samples stored at ultra-low temperature and be transported with dry ice.

Tissues: Animal tissues should not be less than 100 mg, plant tissues should not be less than 500 mg.

Cells: >5x106 cells.

Lysate: The total protein concentration of cell or tissue lysate must be greater than 1 mg/mL, and a single sample should not be less than 50 μL.


Deliverables

WB test data and project report with detailed protocol.

Western Blot Services


WB (Western blot), namely Western blotting or immunoblotting, is a technique widely used in molecular biology, biochemistry, immunology, and many other fields. The method involves using gel electrophoresis to separate the sample's proteins. The separated proteins are transferred out of the gel to the surface of a membrane. The membrane is exposed to an antibody specific to the target protein. Binding of the antibody is detected using a radioactive or chemical tag.

WB technology has the high resolution of SDS-PAGE and the high specificity and sensitivity of solid-phase immunoassays. The selection of a suitable internal reference antibody can correct the errors in protein quantification and sample loading, and the protein expression in different samples can be qualitatively or quantitatively analyzed by grayscale measurement.

Seattle Genova provides WB detection technology services, providing conclusive evidence of the expression level of encoded genes, which can be used for specific gene regulation and gene function research, and can also be used as a verification experiment for protein chips and protein profiles.


Workflow of our service

-Receipt of orders and samples

-Protein concentration determination

-Electrophoretic transfer

-Hybridization

-Chromogenic data analysis

-Result delivery


Sample requirements

Tissue or cell samples stored at ultra-low temperature and be transported with dry ice.

Tissues: Animal tissues should not be less than 100 mg, plant tissues should not be less than 500 mg.

Cells: >5x106 cells.

Lysate: The total protein concentration of cell or tissue lysate must be greater than 1 mg/mL, and a single sample should not be less than 50 μL.


Deliverables

WB test data and project report with detailed protocol.

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