Transwell Migration Assay
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Transwell Migration Assay


The transwell migration assay is a commonly used test to study the migratory response of endothelial cells to angiogenic inducers or inhibitors. This assay is also known as the Boyden or modified Boyden chamber assay. It used the migration of endothelial cells along a cytokine gradient (chemotaxis) to measure the chemotactic capability of test substances in vitro. It involves two medium-filled compartments separated by a microporous membrane. As a rule, cells are placed in the upper compartment and allowed to migrate through the pores of the membrane into the lower compartment, where chemotactic agents are present. After an appropriate incubation time, the membrane between the two compartments is fixed and the number of cells that have migrated to the lower side is determined.


Workflow of Our Service

Receiving orders and samples

Balance chamber

Cell suspension configuration

Cell culture solution configuration

Add solution

Observation, staining, photographing

Results delivered to customers

Sample Requirements

Frozen cells transported with dry ice.

Drugs for the testing.

Deliverables

Original experimental data, pictures, analysis results, experimental process and a complete report.

Transwell Migration Assay


The transwell migration assay is a commonly used test to study the migratory response of endothelial cells to angiogenic inducers or inhibitors. This assay is also known as the Boyden or modified Boyden chamber assay. It used the migration of endothelial cells along a cytokine gradient (chemotaxis) to measure the chemotactic capability of test substances in vitro. It involves two medium-filled compartments separated by a microporous membrane. As a rule, cells are placed in the upper compartment and allowed to migrate through the pores of the membrane into the lower compartment, where chemotactic agents are present. After an appropriate incubation time, the membrane between the two compartments is fixed and the number of cells that have migrated to the lower side is determined.


Workflow of Our Service

Receiving orders and samples

Balance chamber

Cell suspension configuration

Cell culture solution configuration

Add solution

Observation, staining, photographing

Results delivered to customers

Sample Requirements

Frozen cells transported with dry ice.

Drugs for the testing.

Deliverables

Original experimental data, pictures, analysis results, experimental process and a complete report.

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