Soft Agar Colony Formation Assay
  • OVERVIEW

Soft Agar Colony Formation Assay


Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. The assay evaluates the proliferative capacity of a single cell. For applications such as cancer drug screening, it is important to distinguish cells that retain this proliferative capacity from those that do not.

The colony is defined to consist of at least 50 cells. Before or after treatment, cells are seeded out in appropriate dilutions to form colonies in 1–3 weeks. Colonies are fixed with glutaraldehyde (6.0% v/v), stained with crystal violet (0.5% w/v) and counted using a stereomicroscope.


Workflow of Our Service

Receiving orders and samples

Single-cell suspension preparation

Cell seeding and culture

Observing clones

Giemsa staining to calculate clone formation

Results delivered to customers

Sample Requirements

Sample information (including source, full name, etc.).

A well-grown cell line (which can be cryopreserved) for your test, and please provide cell characteristics, culture protocol and related precautions.

Customers need to provide the drugs they want to test.


Deliverables

Original experimental data, pictures, analysis results, our protocol and a complete report.

Soft Agar Colony Formation Assay


Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. The assay evaluates the proliferative capacity of a single cell. For applications such as cancer drug screening, it is important to distinguish cells that retain this proliferative capacity from those that do not.

The colony is defined to consist of at least 50 cells. Before or after treatment, cells are seeded out in appropriate dilutions to form colonies in 1–3 weeks. Colonies are fixed with glutaraldehyde (6.0% v/v), stained with crystal violet (0.5% w/v) and counted using a stereomicroscope.


Workflow of Our Service

Receiving orders and samples

Single-cell suspension preparation

Cell seeding and culture

Observing clones

Giemsa staining to calculate clone formation

Results delivered to customers

Sample Requirements

Sample information (including source, full name, etc.).

A well-grown cell line (which can be cryopreserved) for your test, and please provide cell characteristics, culture protocol and related precautions.

Customers need to provide the drugs they want to test.


Deliverables

Original experimental data, pictures, analysis results, our protocol and a complete report.

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