SDS-PAGE & Silver Staining Service
  • OVERVIEW

SDS-PAGE & Silver Staining Service

Polyacrylamide gel electrophoresis (PAGE) is a commonly used technique for analysis of proteins because of its low cost, ease of use, and high sensitivity. Following one-dimensional or two-dimensional electrophoresis of protein mixtures on a gel, the proteins are typically visualized by some form of protein staining. Over the past several decades, demand for improved sensitivity for small sample sizes and compatibility with downstream applications and detection instrumentation have driven the development of several basic staining methods. Silver protein stains is an ultra-sensitive method for monochromatic detection of proteins in 1D or 2D polyacrylamide gels with metallic silver ions (Ag).

Highlights of silver stains

· Most sensitive colorimetric method for detecting total protein, nanogram-level sensitivity can be achieved.

· Gel compatible—staining performance is very good with a variety of commercial precast gels, handcast gels and SDS-PAGE buffer-systems.

Workflow of Our Service

1.       Validation of samples

2.       SDS-PAGE

3.       Silver staining

4.       Imaging and data processing

5.       Report draft and delivery to customers

SDS-PAGE & Silver Staining Service

Polyacrylamide gel electrophoresis (PAGE) is a commonly used technique for analysis of proteins because of its low cost, ease of use, and high sensitivity. Following one-dimensional or two-dimensional electrophoresis of protein mixtures on a gel, the proteins are typically visualized by some form of protein staining. Over the past several decades, demand for improved sensitivity for small sample sizes and compatibility with downstream applications and detection instrumentation have driven the development of several basic staining methods. Silver protein stains is an ultra-sensitive method for monochromatic detection of proteins in 1D or 2D polyacrylamide gels with metallic silver ions (Ag).

Highlights of silver stains

· Most sensitive colorimetric method for detecting total protein, nanogram-level sensitivity can be achieved.

· Gel compatible—staining performance is very good with a variety of commercial precast gels, handcast gels and SDS-PAGE buffer-systems.

Workflow of Our Service

1.       Validation of samples

2.       SDS-PAGE

3.       Silver staining

4.       Imaging and data processing

5.       Report draft and delivery to customers

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