Real-time Quantitative PCR Services
  • OVERVIEW

Real-time Quantitative PCR Services


Real-time quantitative PCR (real-time quantitative PCR)/qRT-PCR refers to adding fluorescent markers to the PCR reaction system, using the accumulation of fluorescent signals to monitor the entire PCR process in real time, and finally quantitatively analyzing the unknown template through the amplification curve. It is generally used for quantitative research of mRNA transcription level, determination of DNA copy number in different samples or genomes, verification of gene chip results, drug efficacy analysis, etc. Real-time PCR has become one of the most widely used methods of gene quantitation because it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, has little to no post-amplification processing, and is amenable to increasing sample throughput.


Applications

Confirmatory testing for NGS data

Copy Number Variation (CNV) analysis

Genotyping and allelic discrimination

miRNA analysis

SNP genotyping

Nucleic acid and CpG methylation analysis

Plasmid quantification

Pathogen detection and quantification

Service Description

The service includes RNA extraction and fluorescence quantitative PCR detection. The probe method or dye method can be selected.

Service Process Order Receiving and Sample RNA Extraction RNA Quality Testing Sample cDNA Synthesis Quantitative Test Data Analysis Result Delivery After-sales Service Customer Provided

Samples and background information as detailed as possible (such as species information, NCBI accession number of the amplified target gene, relevant literature and sample sources, etc.).


Sample Requirements

Tissue should not be less than 100 mg.

Cells should be 106-107 cells.

Total RNA concentration of cell or tissue lysate should be greater than 1 mg/mL, and a single sample should not be less than 50 μL.

cDNA should not be less than 30 μg.


Deliverables

Dissolution curve, Ct value and complete experimental report.

The content of the experimental report includes the concentration of extracted RNA, the original data of real-time PCR and analysis results.

The complete experimental steps of fluorescence quantitative PCR, the use of instruments and software to set parameters, etc.

Real-time Quantitative PCR Services


Real-time quantitative PCR (real-time quantitative PCR)/qRT-PCR refers to adding fluorescent markers to the PCR reaction system, using the accumulation of fluorescent signals to monitor the entire PCR process in real time, and finally quantitatively analyzing the unknown template through the amplification curve. It is generally used for quantitative research of mRNA transcription level, determination of DNA copy number in different samples or genomes, verification of gene chip results, drug efficacy analysis, etc. Real-time PCR has become one of the most widely used methods of gene quantitation because it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, has little to no post-amplification processing, and is amenable to increasing sample throughput.


Applications

Confirmatory testing for NGS data

Copy Number Variation (CNV) analysis

Genotyping and allelic discrimination

miRNA analysis

SNP genotyping

Nucleic acid and CpG methylation analysis

Plasmid quantification

Pathogen detection and quantification

Service Description

The service includes RNA extraction and fluorescence quantitative PCR detection. The probe method or dye method can be selected.

Service Process Order Receiving and Sample RNA Extraction RNA Quality Testing Sample cDNA Synthesis Quantitative Test Data Analysis Result Delivery After-sales Service Customer Provided

Samples and background information as detailed as possible (such as species information, NCBI accession number of the amplified target gene, relevant literature and sample sources, etc.).


Sample Requirements

Tissue should not be less than 100 mg.

Cells should be 106-107 cells.

Total RNA concentration of cell or tissue lysate should be greater than 1 mg/mL, and a single sample should not be less than 50 μL.

cDNA should not be less than 30 μg.


Deliverables

Dissolution curve, Ct value and complete experimental report.

The content of the experimental report includes the concentration of extracted RNA, the original data of real-time PCR and analysis results.

The complete experimental steps of fluorescence quantitative PCR, the use of instruments and software to set parameters, etc.

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