High Resolution HLA typing
  • OVERVIEW

High Resolution HLA typing


HLA antigens are cell membrane glycoproteins with key roles in the initiation of the immune response. The classical HLA genes can be subdivided into two major classes: HLA class I (HLA-A, B, C) and HLA class II (HLA-DR, DQ, DP) genes. Cell membrane products of these genes are expressed on most cells of the body and present either intra cellular derived peptides or extra cellular derived peptides to the immune system. The diversity of HLA molecules is the result of nucleotide substitutions at exon(s) and or intron(s) sequences. These substitutions can give rise to amino acid differences that may have impact on peptide presentation or interaction with the T-cell receptor.

Current methods for HLA typing define HLA alleles and allele groups using DNA-based methods. HLA typing by next-generation sequencing (NGS) generates unambiguous, phase-resolved HLA typing results using a single assay, system, and analysis program.


Tests

  • HLA-A
  • HLA-B
  • HLA-C
  • HLA-DRB1
  • HLA-DRB3/4/5
  • HLA-DQA1
  • HLA-DQB1
  • HLA-DPA1
  • HLA-DPB1


Highlights

  • Next-generation sequencing (NGS) is used to determine the whole HLA gene sequences including all exons and introns.
  • Comprehensive NGS typing includes all eleven major histocompatibility HLA genes: HLA-A, B, C, DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, and DPB1.
  • HLA typing results are reported up to three-digit format (e.g., HLA-A*01:01:01).

High Resolution HLA typing


HLA antigens are cell membrane glycoproteins with key roles in the initiation of the immune response. The classical HLA genes can be subdivided into two major classes: HLA class I (HLA-A, B, C) and HLA class II (HLA-DR, DQ, DP) genes. Cell membrane products of these genes are expressed on most cells of the body and present either intra cellular derived peptides or extra cellular derived peptides to the immune system. The diversity of HLA molecules is the result of nucleotide substitutions at exon(s) and or intron(s) sequences. These substitutions can give rise to amino acid differences that may have impact on peptide presentation or interaction with the T-cell receptor.

Current methods for HLA typing define HLA alleles and allele groups using DNA-based methods. HLA typing by next-generation sequencing (NGS) generates unambiguous, phase-resolved HLA typing results using a single assay, system, and analysis program.


Tests

  • HLA-A
  • HLA-B
  • HLA-C
  • HLA-DRB1
  • HLA-DRB3/4/5
  • HLA-DQA1
  • HLA-DQB1
  • HLA-DPA1
  • HLA-DPB1


Highlights

  • Next-generation sequencing (NGS) is used to determine the whole HLA gene sequences including all exons and introns.
  • Comprehensive NGS typing includes all eleven major histocompatibility HLA genes: HLA-A, B, C, DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, and DPB1.
  • HLA typing results are reported up to three-digit format (e.g., HLA-A*01:01:01).
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