Dual-Luciferase Reporter (DLR) Assay
  • OVERVIEW

Dual-Luciferase Reporter (DLR) Assay


Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalizing transfection control for firefly luciferase assays.

Dual Reporter Assay or dual luciferase assay is most performed with firefly and renilla luciferase. The dual system improves your overall accuracy by normalizing your data. In this system, one reporter (e.g. firefly luciferase) reveals the target promoter activity while the other (e.g. renilla luciferase) is used as your control for transfection efficiency. Therefore, in this experiment, your green firefly luciferase is going to measure experimental conditions, while your blue renilla luciferase relates to a constitutive promoter, measuring transfection and cell viability.


Highlights of the Dual Reporter Assay

-Sensitive –highly sensitive detection of firefly luciferase activity and Renilla luciferase activity

-Stable –the luciferase signal output is stable for more than one hour, providing flexibility about incubation time

-High-throughput –simple homogeneous protocol minimizes handling steps to support high-throughput screening applications

-Compatibility –works well with a variety of common media containing 0-10% serum and phenol red


Workflow of Our Service

-Sample delivery

-Sequence analysis

-Synthesis or amplification of target gene and subcloning into target vectors

-Co-transfection of DLR plasmids in HEK293T cells

-Luciferase luminescence

-Data analysis and project report writing


Starting Material

Plasmids with your target gene

Digital sequence of the genes of interest

Information about the transcription factors


Deliverables

Project report, including the original experimental data, pictures, analysis results, etc.

Dual-Luciferase Reporter (DLR) Assay


Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. It commonly is used in multiplex transcriptional reporter assays or as a normalizing transfection control for firefly luciferase assays.

Dual Reporter Assay or dual luciferase assay is most performed with firefly and renilla luciferase. The dual system improves your overall accuracy by normalizing your data. In this system, one reporter (e.g. firefly luciferase) reveals the target promoter activity while the other (e.g. renilla luciferase) is used as your control for transfection efficiency. Therefore, in this experiment, your green firefly luciferase is going to measure experimental conditions, while your blue renilla luciferase relates to a constitutive promoter, measuring transfection and cell viability.


Highlights of the Dual Reporter Assay

-Sensitive –highly sensitive detection of firefly luciferase activity and Renilla luciferase activity

-Stable –the luciferase signal output is stable for more than one hour, providing flexibility about incubation time

-High-throughput –simple homogeneous protocol minimizes handling steps to support high-throughput screening applications

-Compatibility –works well with a variety of common media containing 0-10% serum and phenol red


Workflow of Our Service

-Sample delivery

-Sequence analysis

-Synthesis or amplification of target gene and subcloning into target vectors

-Co-transfection of DLR plasmids in HEK293T cells

-Luciferase luminescence

-Data analysis and project report writing


Starting Material

Plasmids with your target gene

Digital sequence of the genes of interest

Information about the transcription factors


Deliverables

Project report, including the original experimental data, pictures, analysis results, etc.

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