Co-Immunoprecipitation Service
  • OVERVIEW

Co-Immunoprecipitation Service


Immunoprecipitation (IP), a method using a target protein-specific antibody in conjunction with Protein A/G affinity beads, is a powerful tool to identify molecules that interact with specific proteins. Co-immunoprecipitation is similar to immunoprecipitation studies in which a specific protein can be isolated from a solution containing many other proteins such as from eukaryotic or prokaryotic whole-cell lysates. Immunoprecipitation studies typically require that the protein-specific antibody be bound to a solid substrate or resin allowing for the protein to be precipitated from the solution. Co-immunoprecipitation experiments are performed in a similar manner, but co-immunoprecipitation refers to the fact that proteins tightly bound to the antibody-specific protein will also be precipitated or “pulled-down” in the experimental assay.


Workflow

-Receipt of orders and samples

-Sample lysate preparation, quantification

-Sample, antibody, protein A/G-gel incubation

-Pellets collection, washing, sample preparation

-WB detection

-Result delivery


Sample requirements

Tissue or cell samples that are stored at ultra-low temperature and be transported with dry ice.

Tissue: animal tissues not less than 400 mg, plant tissue not less than 2g.

Cells: >2x107 cells.


Deliverables

WB data and project report.

Co-Immunoprecipitation Service


Immunoprecipitation (IP), a method using a target protein-specific antibody in conjunction with Protein A/G affinity beads, is a powerful tool to identify molecules that interact with specific proteins. Co-immunoprecipitation is similar to immunoprecipitation studies in which a specific protein can be isolated from a solution containing many other proteins such as from eukaryotic or prokaryotic whole-cell lysates. Immunoprecipitation studies typically require that the protein-specific antibody be bound to a solid substrate or resin allowing for the protein to be precipitated from the solution. Co-immunoprecipitation experiments are performed in a similar manner, but co-immunoprecipitation refers to the fact that proteins tightly bound to the antibody-specific protein will also be precipitated or “pulled-down” in the experimental assay.


Workflow

-Receipt of orders and samples

-Sample lysate preparation, quantification

-Sample, antibody, protein A/G-gel incubation

-Pellets collection, washing, sample preparation

-WB detection

-Result delivery


Sample requirements

Tissue or cell samples that are stored at ultra-low temperature and be transported with dry ice.

Tissue: animal tissues not less than 400 mg, plant tissue not less than 2g.

Cells: >2x107 cells.


Deliverables

WB data and project report.

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