Chromosome Analysis
  • OVERVIEW


Chromosome analysis


Chromosome analysis is the microscopic examination of chromosomes in the metaphase stage of the cell cycle. This type of analysis detects changes of the chromosome modal number and within the chromosome structure. These changes include trisomies, monosomies, deletions and balanced or unbalanced translocations.


G Banding
During mitosis, the 23 pairs of human chromosomes condense and are visible with a light microscope. A karyotype analysis usually involves blocking cells in mitosis and staining the condensed chromosomes with Giemsa dye. The dye stains regions of chromosomes that are rich in the base pairs Adenine (A) and Thymine (T) producing a dark band. The analysis involves comparing chromosomes for their length, the placement of centromeres (areas where the two chromatids are joined), and the location and sizes of G-bands.

Multi color FISH
FISH is a laboratory technique used to determine how many copies of a specific segment of DNA are present in a cell. It is also used to identify structurally-abnormal chromosomes. In the lab, a segment of DNA is chemically modified and labeled so that it will look fluorescent (very brightly colored) under microscope. This DNA is called a "probe." Probes can find matching segments of DNA when added to cells under certain conditions. A FISH study does not replace a chromosome study but is done in addition to a standard chromosome study, depending on the customers’ requests.


Number of cells analyzed:
- 10 cells analyzed per sample
- 1 cell analyzed per cancer/highly abnormal sample

Sample Requirement
We accept both human and mouse fixed cells

Chromosome prototype can be displayed
Unclassified chromosomes can be automatically corrected, or manually if desired, for the following:
– Superimpositions: A chromosome appears superimposed to other in one part
– Separation: When two chromosomes are joint in an extreme, can be automatically
– Crossings
– Straightening
– Rotations
– Flip a chromosome
– Obtaining the specular image, as well as Magnification of the individual chromosomes is available
– Best Brightness and contrast can be modified and automatically obtained
– Damaged chromosomes bands can be highlighted in the karyotype study



Chromosome analysis


Chromosome analysis is the microscopic examination of chromosomes in the metaphase stage of the cell cycle. This type of analysis detects changes of the chromosome modal number and within the chromosome structure. These changes include trisomies, monosomies, deletions and balanced or unbalanced translocations.


G Banding
During mitosis, the 23 pairs of human chromosomes condense and are visible with a light microscope. A karyotype analysis usually involves blocking cells in mitosis and staining the condensed chromosomes with Giemsa dye. The dye stains regions of chromosomes that are rich in the base pairs Adenine (A) and Thymine (T) producing a dark band. The analysis involves comparing chromosomes for their length, the placement of centromeres (areas where the two chromatids are joined), and the location and sizes of G-bands.

Multi color FISH
FISH is a laboratory technique used to determine how many copies of a specific segment of DNA are present in a cell. It is also used to identify structurally-abnormal chromosomes. In the lab, a segment of DNA is chemically modified and labeled so that it will look fluorescent (very brightly colored) under microscope. This DNA is called a "probe." Probes can find matching segments of DNA when added to cells under certain conditions. A FISH study does not replace a chromosome study but is done in addition to a standard chromosome study, depending on the customers’ requests.


Number of cells analyzed:
- 10 cells analyzed per sample
- 1 cell analyzed per cancer/highly abnormal sample

Sample Requirement
We accept both human and mouse fixed cells

Chromosome prototype can be displayed
Unclassified chromosomes can be automatically corrected, or manually if desired, for the following:
– Superimpositions: A chromosome appears superimposed to other in one part
– Separation: When two chromosomes are joint in an extreme, can be automatically
– Crossings
– Straightening
– Rotations
– Flip a chromosome
– Obtaining the specular image, as well as Magnification of the individual chromosomes is available
– Best Brightness and contrast can be modified and automatically obtained
– Damaged chromosomes bands can be highlighted in the karyotype study


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