Plasmid DNA Production
  • OVERVIEW

Plasmid DNA Production

Seattle Genova’s Plasmid DNA Production services provide high quantity transfection grade plasmid DNA from bacterial cultures. Choose from a variety of our packages to help you achieve highly efficient cell transfection. Our plasmid DNA preparation process is designed to meet our customers’ diverse downstream applications such as transfection, antibody preparation, vaccine, and gene-therapy research, etc.

We take flexible sample formats such as agar plates, stripes, glycerol stock and liquid cultures. Featuring an innovative magnetic beads purification system, the service turnaround time is 50% faster than conventional methods using spin/vacuum columns.


Endotoxin free plasmid DNA preparation

We also provide our rigorous quality controlled endotoxin removal procedure in producing endotoxin free plasmid DNA for customers who work with direct transfection with mammalian cell lines or stem cells. With our guaranteed endotoxin free standard (<0.05 EU/ug plasmid DNA, 20 times cleaner than most other service providers), you can achieve high expression levels of the plasmid with minimal worry regarding the cell toxicity.


Animal-free plasmid DNA preparation

We offer animal-products free plasmid DNA preparation services to provide you with plasmids that are free of transmissible spongiform encephalopathies (TSE) including bovine spongiform encephalopathy (BSE). Our production process has been optimized so no animal-derived components are used during manufacturing. The culture media we use consists of a plant-based tryptone substitute which replaces the traditional bovine-derived tryptone. Our DNA purification is conducted via non-enzymatic processes to circumvent the usage of RNase. The plasmid preparation is performed at separate lab stations by trained technicians to ensure the plasmids are free of contamination.


QC Standard:

260/280

1.80-2.00

Appearance

Clear and colorless

Sterility assurance

No growth on LB plate in 48 hr

DNA-Homogeneity

Predominantly supercoiled

Endotoxin (EU/mg)

< 5

Genomic DNA

less than 1%

Identity

Gel analysis/ restriction enzyme/ DNA sequencing verification

RNA contamination

Not visible on agarose gel


Competitive Advantages:

· USA based manufacturing allows high quality products and fast delivery.

· Highly Customized: Microgram-to-gram-scale quantity can meet various customers’ needs.

· Low Endotoxin Level

· Animal-derived materials free


Deliverables:

· Entire amount of purified plasmid

· Project Report

· DNA sequencing data

Plasmid DNA Production

Seattle Genova’s Plasmid DNA Production services provide high quantity transfection grade plasmid DNA from bacterial cultures. Choose from a variety of our packages to help you achieve highly efficient cell transfection. Our plasmid DNA preparation process is designed to meet our customers’ diverse downstream applications such as transfection, antibody preparation, vaccine, and gene-therapy research, etc.

We take flexible sample formats such as agar plates, stripes, glycerol stock and liquid cultures. Featuring an innovative magnetic beads purification system, the service turnaround time is 50% faster than conventional methods using spin/vacuum columns.


Endotoxin free plasmid DNA preparation

We also provide our rigorous quality controlled endotoxin removal procedure in producing endotoxin free plasmid DNA for customers who work with direct transfection with mammalian cell lines or stem cells. With our guaranteed endotoxin free standard (<0.05 EU/ug plasmid DNA, 20 times cleaner than most other service providers), you can achieve high expression levels of the plasmid with minimal worry regarding the cell toxicity.


Animal-free plasmid DNA preparation

We offer animal-products free plasmid DNA preparation services to provide you with plasmids that are free of transmissible spongiform encephalopathies (TSE) including bovine spongiform encephalopathy (BSE). Our production process has been optimized so no animal-derived components are used during manufacturing. The culture media we use consists of a plant-based tryptone substitute which replaces the traditional bovine-derived tryptone. Our DNA purification is conducted via non-enzymatic processes to circumvent the usage of RNase. The plasmid preparation is performed at separate lab stations by trained technicians to ensure the plasmids are free of contamination.


QC Standard:

260/280

1.80-2.00

Appearance

Clear and colorless

Sterility assurance

No growth on LB plate in 48 hr

DNA-Homogeneity

Predominantly supercoiled

Endotoxin (EU/mg)

< 5

Genomic DNA

less than 1%

Identity

Gel analysis/ restriction enzyme/ DNA sequencing verification

RNA contamination

Not visible on agarose gel


Competitive Advantages:

· USA based manufacturing allows high quality products and fast delivery.

· Highly Customized: Microgram-to-gram-scale quantity can meet various customers’ needs.

· Low Endotoxin Level

· Animal-derived materials free


Deliverables:

· Entire amount of purified plasmid

· Project Report

· DNA sequencing data

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