Antibiotic Switching Service
  • OVERVIEW

Antibiotic Switching Service

Seattle Genova offers Antibiotic Gene Switching Services to our customers. Starting from any vector, we can either add an antibiotic gene to the vector or we can switch the existing antibiotic gene to another one.

Many plasmids are designed to include an antibiotic resistance gene, which when expressed, allows only plasmid-containing bacteria to grow in or on media containing that antibiotic. These antibiotic resistance genes not only give the scientist with an easy way to detect plasmid-containing bacteria, but also provide those bacteria with a pressure to maintain and replicate your plasmid over multiple generations.

Below you will find a few antibiotics commonly used in the lab and their recommended concentrations. We suggest checking your plasmid's datasheet or the plasmid map to confirm which antibiotic(s) to add to your LB media or LB agar plates.

Name

Class

Mode of Action

Working Concentration

Kanamycin

aminoglycoside

Binds 30S ribosomal subunit; causes mis-translation

50-100 ug/mL

Spectinomycin

aminoglycoside

Binds 30S ribosomal subunit; interrupts protein synthesis

7.5-50 ug/mL

Streptomycin

aminoglycoside

Inhibits initiation of protein synthesis

25-100 ug/mL

Ampicillin

beta-lactam

Inhibits cell wall synthesis

100-200 ug/mL

Carbenicillin

beta-lactam

Inhibits cell wall synthesis

100 ug/mL

Bleomycin

glycopeptide

Induces DNA breaks

5-100 ug/mL

Erythromycin

macrolide

Blocks 50S ribosomal subunit; inhibits aminoacyl translocation

50-100 ug/mL in EtOH

Polymyxin B

polypeptide

Alters outer membrane permeability

10-100 ug/mL

Tetracycline

tetracyclin

Binds 30S ribosomal subunit; inhibits protein synthesis (elongation step)

10 ug/mL

Chloramphenicol

Binds 50S ribosomal subunit; inhibits peptidyl translocation

5-25 ug/mL in EtOH


Deliverables

· 10 μg of engineered plasmid

· Project report including primer design and reporter sequences and cloning methods

· Sequencing data

Antibiotic Switching Service

Seattle Genova offers Antibiotic Gene Switching Services to our customers. Starting from any vector, we can either add an antibiotic gene to the vector or we can switch the existing antibiotic gene to another one.

Many plasmids are designed to include an antibiotic resistance gene, which when expressed, allows only plasmid-containing bacteria to grow in or on media containing that antibiotic. These antibiotic resistance genes not only give the scientist with an easy way to detect plasmid-containing bacteria, but also provide those bacteria with a pressure to maintain and replicate your plasmid over multiple generations.

Below you will find a few antibiotics commonly used in the lab and their recommended concentrations. We suggest checking your plasmid's datasheet or the plasmid map to confirm which antibiotic(s) to add to your LB media or LB agar plates.

Name

Class

Mode of Action

Working Concentration

Kanamycin

aminoglycoside

Binds 30S ribosomal subunit; causes mis-translation

50-100 ug/mL

Spectinomycin

aminoglycoside

Binds 30S ribosomal subunit; interrupts protein synthesis

7.5-50 ug/mL

Streptomycin

aminoglycoside

Inhibits initiation of protein synthesis

25-100 ug/mL

Ampicillin

beta-lactam

Inhibits cell wall synthesis

100-200 ug/mL

Carbenicillin

beta-lactam

Inhibits cell wall synthesis

100 ug/mL

Bleomycin

glycopeptide

Induces DNA breaks

5-100 ug/mL

Erythromycin

macrolide

Blocks 50S ribosomal subunit; inhibits aminoacyl translocation

50-100 ug/mL in EtOH

Polymyxin B

polypeptide

Alters outer membrane permeability

10-100 ug/mL

Tetracycline

tetracyclin

Binds 30S ribosomal subunit; inhibits protein synthesis (elongation step)

10 ug/mL

Chloramphenicol

Binds 50S ribosomal subunit; inhibits peptidyl translocation

5-25 ug/mL in EtOH


Deliverables

· 10 μg of engineered plasmid

· Project report including primer design and reporter sequences and cloning methods

· Sequencing data

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