BioActive™ Human Noggin
Bioactive™ Recombinant Human Noggin
Catalog Number: SG1840N
Source:
Human Embryonic Kidney 293 cell expressed human Noggin protein
Gln28-Cys232
Accession # Q13253
Request Quote
First Name *
Last Name *
Email Address *
Instiution *
Prodcuts/Services *
Questions & Comments *
Download
BioActive™ Human Noggin

Bioactive Recombinant Human Noggin

Catalog Number: SG1840N

DESCRIPTION

Source

Human Embryonic Kidney 293 cell expressed human Noggin protein

Gln28-Cys232

Accession # Q13253

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

23 kDa (monomer)

SPECIFICATIONS

SDS-PAGE

30-33 kDa, reducing conditions

Activity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.

The ED50 for this effect is 0.02-0.16 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP4.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE with silver staining, under reducing conditions.

Host Cell Protein

< 1.0 ng per μg of protein when tested by ELISA.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.

PREPARATION AND STORAGE

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.

1 month, 2 to 8 °C under sterile conditions.

3 months, ≤ -20 °C under sterile conditions.


Bioactive Recombinant Human Noggin

Catalog Number: SG1840N

DESCRIPTION

Source

Human Embryonic Kidney 293 cell expressed human Noggin protein

Gln28-Cys232

Accession # Q13253

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

23 kDa (monomer)

SPECIFICATIONS

SDS-PAGE

30-33 kDa, reducing conditions

Activity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.

The ED50 for this effect is 0.02-0.16 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP4.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE with silver staining, under reducing conditions.

Host Cell Protein

< 1.0 ng per μg of protein when tested by ELISA.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.

PREPARATION AND STORAGE

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.

1 month, 2 to 8 °C under sterile conditions.

3 months, ≤ -20 °C under sterile conditions.